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Sample information : Glioma



Estimated Number of Cells
The total number of barcodes identified as cells.
Mean Reads per Cell
The number of reads that mapped to a unique gene in the transcriptome divided by the number of cell-associated barcodes.
Median UMI Counts per Cell
The median number of UMI counts per %s cell-associated barcodes.
Median Genes per Cell
The median number of genes detected per cell-associated barcodes.
9,065

Estimated Number of Cells

9,890

Mean reads per Cell

3,397

Median UMI Counts per Cell

1,735

Median Genes per Cell

Cells


Estimated Number of Cells
The total number of barcodes identified as cells.
Fraction Reads in cells
The fraction of valid-barcode, confidently-mapped reads with cell-associated barcodes.
Mean Reads per Cell
The number of reads that mapped to a unique gene in the transcriptome divided by the number of cell-associated barcodes.
Median UMI Counts per Cell
The median number of UMI counts per %s cell-associated barcodes.
Median Genes per Cell
The median number of genes detected per cell-associated barcodes.
Total Genes Detected
The number of genes with at least one UMI count in any cells.
Barcode Rank Plot
The plot shows the count of UMIs mapped to each barcode.
Sample Name
Glioma
Species
Human
Type
FFPE
Organ
Glioma
Estimated Number of Cells
9,065
Fraction Reads in cells
60.46%
Mean Reads per Cell
9,890
Median UMI Counts per Cell
3,397
Median genes per cell
1,735
Total genes detected
33,281
Sequencing & Mapping


Number of Reads
Total number of read pairs.
Valid Barcodes
Fraction of reads with barcodes that match the whitelist after barcode correction.
Valid UMIs
Fraction of reads with valid UMIs; i.e. UMI sequences that do not contain Ns and that are not homopolymers.
Sequencing Saturation
The fraction of reads originating from an already-observed UMI. This is a function of library complexity and sequencing depth. More specifically, this is the fraction of confidently mapped, valid cell-barcode, valid UMI reads that had a non-unique (cell-barcode, UMI, gene).
Q30 Bases in RNA Read
Fraction of RNA read bases with Q-score ≥ 30.
Reads Mapped to Genome
Fraction of reads that mapped to the genome.
Reads Mapped Confidently to Genome
Fraction of reads that mapped uniquely to the genome.
Reads Mapped Confidently to Transcriptome
Fraction of reads that mapped to a unique gene in the transcriptome. These reads are considered for UMI counting.
Reads Mapped to Exonic Regions
Fraction of reads that mapped to an exonic region of the genome.
Reads Mapped to Intronic Regions
Fraction of reads that mapped to an intronic region of the genome.
Reads Mapped to LncRNA Regions
Fraction of reads that mapped to a lncRNA region of the genome.
Sequencing
Number of Reads
233,196,168
Valid Barcodes
93.33%
Valid UMIs
100.0%
Sequencing Saturation
56.9%
Q30 Bases in RNA Read
91.14%
Mapping
Reads Mapped to Genome
90.94%
Reads Mapped Confidently to Genome
79.22%
Reads Mapped Confidently to Transcriptome
69.84%
Reads Mapped to Exonic Regions
11.88%
Reads Mapped to Intronic Regions
59.59%
Reads Mapped to LncRNA Regions
8.32%
Cluster


Left
The figure is automated clustering each cell-barcode by UMAP algorithm. The cells clustered into the same group have similar expression profiles. Each dot represents a cell, and is colored according to different cluster.
Right
This plot shows that the total UMI counts for each cell-barcode. Two-dimensional horizontal and vertical coordinates of each dot are obtained using the uniform manifold approximation and projection (UMAP) algorithm. Each dot represents a cell and is colored according to UMI counts. Cells with greater UMI counts likely have higher RNA content than cells with fewer ones.
Marker


Table
The differential expression analysis seeks to find, for each cluster, features that are more highly expressed in that cluster relative to the rest of the sample. Here a differential expression test was performed between each cluster and the rest of the sample for each feature. The Log2 fold-change (L2FC) is an estimate of the log2 ratio of expression in a cluster to that in all other cells. A value of 1.0 indicates 2-fold greater expression in the cluster of interest. The p-value is been adjusted via the Benjamini-Hochberg procedure reported as p_val_adj. The pct.1 is the percentage of cells where the feature is detected in that cluster. And the pct.2 is the percentage of cells where the feature is detected in the rest of the sample. The features were filtered by p_value < 0.01, p_val_adj < 0.05, pct.1 ≥ 0.1, pct.1 ≥ 0.1, and avg_log2FC > 0.25 for each cluster were retained. In this table you can click on a column to sort by that value.
gene cluster p_val_adj p_val avg_log2FC pct.1 pct.2
FGF1213.6108886734393e-2441.39832268653499e-2481.18602335657090.9610.796
CCDC2612.83578629920263e-2461.09816299392117e-2501.171507912132020.920.658
LHFPL311.27578824134832e-2854.94051133233288e-2901.080168226158140.9990.948
REV3L15.81410880996922e-2712.25152337449918e-2750.9908115554604750.9930.92
SMOC111.50327036033172e-2115.82143964811107e-2160.9635087641582320.9470.799
DLGAP115.04675499315132e-2271.95436432372355e-2310.9200476256025650.9990.972
MAP216.57215361897889e-2362.54507749640975e-2400.84102911502970.9930.919
PVT116.63389194848367e-1772.56898576791375e-1810.7908065451798050.940.771
CADM218.95519191489161e-1933.46791306776579e-1970.7332628594239010.9990.971
EPHB112.5338709625657e-799.81245774141539e-840.7133525642473160.890.806
MTSS114.34351266701809e-1721.68203255509356e-1760.7080365615449330.9850.852
MEGF1111.74872796063104e-1346.77197831634993e-1390.6948020431763130.9180.732
COL20A115.87462543154103e-1802.27495853755994e-1840.6791330874876480.6160.252
GRIK317.61310728198943e-1402.94818854586587e-1440.671334936839930.9420.787
SCFD218.69728254950695e-1353.3680372340576e-1390.6668020863449830.9040.732
IGSF9B13.66168082923012e-1361.41799203393491e-1400.6564000041900320.6120.29
FAM110B16.00368912796822e-1572.32493867016544e-1610.645015825142990.9750.827
DSCAML118.60238479857554e-1283.33128792106864e-1320.6332185430130670.9880.912
SH3D1911.75033258282918e-1406.77819224268744e-1450.6313902678755870.9270.737
SEZ6L11.67114602215198e-1156.47154096019821e-1200.6247763982289820.970.889
LINC0051111.15755139822372e-1214.48263717702715e-1260.6130670510159770.9070.712
EPN211.8868858701815e-1277.30699713504048e-1320.6116145856025470.8670.65
XKR411.53568288648557e-845.94695769850743e-890.6015554934050590.880.698
LDLRAD317.41988656910515e-1242.87336350118311e-1280.6007798421152120.9650.867
MACROD111.63237115428343e-996.32138463495112e-1040.5786308739316660.7640.53
SCN3A11.99642295278728e-937.73118132202798e-980.5743731504222360.870.697
ST3GAL511.26780004698554e-624.90957691587166e-670.5501414231052340.6140.409
FERMT111.15122299693932e-1204.45813033706123e-1250.5472352051996030.6360.32
ENSG0000025808112.03253306450592e-817.87101833445349e-860.5413801260213640.6570.41
LINC0150512.32996261518582e-529.02281925100035e-570.5363343299347090.5330.342
KHDRBS314.46918798120414e-731.7307005310011e-770.5247728994490530.8210.673
ZNF12417.16084621440513e-722.7730496899683e-760.5214132183723750.5010.268
FGF1412.39810272113061e-569.28669295252531e-610.5150056246739470.8530.735
CMSS115.14873060603242e-871.99385455060699e-910.5107795027750820.8350.659
ARHGAP2412.59714316208342e-121.00574803937708e-160.5058325344848610.4790.403
SIK311.35335759293335e-1045.24089994552667e-1090.5045162538292810.9430.846
ERC217.05103452827095e-992.73052493059325e-1030.4972433293373190.9130.766
GRIA212.78894180017364e-1001.08002238321405e-1040.4910069772235840.9770.882
ENSG0000023342017.54400884552075e-582.92143006061292e-620.488981243046350.3450.165
SOX611.53786026794062e-925.95538964466027e-970.4864056310178780.9570.842
SLC19A114.46703285946251e-1071.72986595649712e-1110.482727179524450.6030.305
CHD717.13523283663748e-1122.76313086652886e-1160.4814382848386690.9680.831
ADAM2218.75770094907465e-713.39143436048277e-750.4735581335227510.8820.749
JMJD1C13.48008576681105e-831.34766904186619e-870.4683934577563870.9860.964
NOVA115.40809292850538e-912.09429304438112e-950.4634180654164950.9870.926
PRKCE15.84957100489337e-502.26525616887789e-540.4541111497357660.870.786
SERINC511.45789419678705e-615.64571969479555e-660.4515793685532350.8110.668
KLRC4-KLRK118.01652629735412e-993.10441323523763e-1030.4481866593807580.5720.292
SMARCC118.0603164160208e-853.12137103203377e-890.4472025506659650.9410.839
HIP111.26628672051626e-614.90371653377322e-660.4427354455407320.8140.66
ROBO122.40926351119193e-2379.32991329896576e-2421.702655343259020.9370.651
CSMD125.34855540852306e-1382.07123704005075e-1421.435838201715540.8590.616
LINGO223.99062232368504e-1391.54537517859468e-1431.397379529130210.6630.322
CLSTN222.96209492021223e-1821.14707621895683e-1861.388943271296230.7670.391
CELF425.17980277383479e-2462.00588729963009e-2501.292465887619140.6130.173
MOB3B22.01602604444795e-1677.80709462280891e-1721.224837656149930.790.453
SOX2-OT21.14087503327988e-1874.41805767447576e-1921.113717633713410.9910.884
GRIK221.53400981166529e-1705.94047868824417e-1751.091628279900310.9750.86
SLIT129.29621195049845e-1193.59997364771655e-1231.066879055128640.6830.356
CACNA1A22.1683056438957e-308.39679992214575e-351.050782296098380.840.819
KCND321.6878730545694e-1186.53631667338961e-1231.04611336064250.7740.479
LINC0200822.69256357489003e-1691.0426997540526e-1731.028015054618760.4530.121
GRIA123.34523566101612e-1161.29544811254158e-1201.020167244768340.7280.4
NEDD4L21.82381744277392e-827.06276359359453e-870.9489911537520070.70.462
BOC21.12873483200672e-2424.37104454171366e-2470.9482580055057920.5680.142
ENSG0000023191826.06559387820427e-542.34891138837636e-580.9231932168487220.7010.536
PAK322.76434158120206e-931.0704959072153e-970.8784610219965590.7360.49
APBA124.55348553191504e-1211.76334489870079e-1250.8740408247766440.7260.411
PBX121.04669253957375e-954.05333439017059e-1000.8711986465615770.8860.71
KCNH723.72678697564059e-1291.44320449817627e-1330.8706326500481130.7060.345
SEZ6L24.57123575231558e-1001.77021870128009e-1040.8655470694599070.9780.894
PTPRN222.93133270915266e-1331.13516350120151e-1370.8576958395730790.9760.849
CTNNA222.17800225514408e-1288.4343502116101e-1330.8394900741361180.9920.942
THSD7A28.57086792923378e-763.31908296062959e-800.8370042161574770.5280.272
SLC24A321.49765422929308e-585.79969108660141e-630.7839372249605290.8160.671
KLHDC8A24.23254984774069e-1921.63906201748081e-1960.7812913291668330.4930.126
PTPRS22.1659824739505e-1398.38780340762304e-1440.7788181385141930.9720.854
SPECC121.73460254266495e-946.71727739869477e-990.749325723588070.5910.293
RIT224.2494630775316e-791.64561169404469e-830.7317212840487810.5410.262
KCNQ321.20558691268872e-564.66865551132215e-610.7149050749246120.8860.76
ADAM2221.10835280745998e-644.29211481028534e-690.7022499971479820.8830.759
ZNF804A22.54706631905629e-179.86355698042944e-220.6810453250561590.4490.335
KIF21A21.17662643187763e-974.55650556433267e-1020.6792140462929160.5790.277
DGKB22.6030138084692e-551.0080214570225e-590.6732682003916260.8350.675
CDON22.94021694795589e-1041.13860393755795e-1080.6704051542195580.550.243
TFDP226.05559781938741e-542.34504039785749e-580.6674879017536980.7260.552
DCX24.5098839946295e-1121.74646013036034e-1160.6640309569076090.460.167
CHD721.87865304849073e-1047.27511539515444e-1090.6631070338798050.9740.841
TOX329.67082988655925e-813.74504507089e-850.6513083649835420.6060.324
PRANCR24.96438767795677e-541.92246744296045e-580.6446235999873920.530.3
NCOR221.97046549048861e-667.6306606145243e-710.6429815942736940.9250.833
IGSF2121.38822213375029e-725.37591346377372e-770.6389992515079010.5730.298
NDUFAF621.13855748499478e-734.40908292992597e-780.6219784937207610.830.62
NFIX21.64149683394473e-696.35672398228218e-740.620323471480460.8050.617
ENSG0000028804125.20045623506861e-762.01388538708462e-800.618834559423810.5460.274
LINC0063922.78362253933747e-1001.07796249054621e-1040.6119819483977520.2510.056
ZNF70429.21848914271899e-563.56987536022886e-600.5893736295268610.8720.755
ZHX225.48030637739521e-462.12225782341138e-500.5834970685248970.8960.794
ROBO221.90417642664164e-467.37395510452557e-510.5780584684724190.6560.447
TCF423.11733135177453e-781.20719178707917e-820.5752766531157310.9730.925
ADGRB13002.936404401665470.7440.17
NKAIN33002.138747394580120.9690.617
ADCY23001.993265178825290.7730.11
EEPD13001.80958820755540.8050.164
LRIG13001.667528963113230.950.554
ADCY832.83603876712929e-2551.09826076254862e-2591.608769670784460.910.503
OPHN132.75625738571685e-2851.06736528897373e-2891.606980864331350.9780.746
DCLK134.29262444799223e-2651.66232600704497e-2691.589700757307010.7320.245
ADGRV13001.54870309420650.7540.221
TNIK3001.436849887371720.7770.225
EXD339.91170990526493e-613.83832626157493e-651.40471037518280.9020.768
FRMD531.63282031490615e-2226.32312401698543e-2271.394887020584050.8710.484
KCNN33001.336829235808840.6290.12
TMEM132B35.86406148578373e-2172.27086763187226e-2211.297373758729650.9590.705
OBI1-AS13001.288003415470990.5860.094
SPON131.80058684133334e-1996.97280270043502e-2041.218321455049320.7560.345
EDNRB31.34155450361854e-2875.19519228446941e-2921.186683262267980.6870.194
DCLK239.00211002025724e-2183.48608218265006e-2221.185581506546980.9370.639
ASTN238.850110168334e-1793.42721998541378e-1831.183811466297290.6680.243
TNC3001.173149932656030.5580.099
PTPRM34.71376528217436e-1561.82541350043541e-1601.125152054246330.6690.28
CHST934.96623911162085e-1641.92318441374776e-1681.089436532409730.7840.395
TACR13001.087938312451120.4950.077
RGMA36.80514055854579e-2422.63530207897835e-2461.029893476622590.6370.187
AKAP631.24283489036553e-1784.81289892872838e-1831.014180400490360.9010.591
MIR9-1HG34.18651882176628e-1571.62123642557653e-1611.005692665816530.960.838
ANOS131.19398968967931e-2654.62374507098058e-2701.000022951790510.5050.094
GLIS333.53169079954536e-1491.36765317722393e-1530.9965764460208570.5930.214
MYO1631.26398812384197e-1604.89481517965367e-1650.9962232834300220.5580.196
ADGRB331.81044284092294e-1497.01097022392031e-1540.9881545884141980.9880.918
LRRC733.04681673542812e-1561.17988488379666e-1600.9756070335951210.3390.068
ENSG0000028367433.06728444385067e-1101.18781103816391e-1140.9578860214152570.7850.504
LIFR36.84959378031359e-2192.65251666356101e-2230.9315835592257030.640.187
KCNH735.19432383223894e-902.01151060381789e-940.9255318204289150.640.355
NALCN39.09077331409635e-1393.52041719168817e-1430.9133602805720380.7580.423
COL22A138.9123128446394e-2433.45130807599404e-2470.9001362741418490.4460.078
SHANK232.02974314225907e-757.86021431382515e-800.8912995720342240.5150.263
ADCYAP1R133.92283322251825e-1651.51912373563035e-1690.890812799706530.5150.157
MSI235.41726709885001e-1422.09784575721257e-1460.8755598567447920.9520.752
SORCS332.0009918741773e-1137.74887454663402e-1180.8721648088648080.7910.487
ENSG0000026401533.52685953247298e-1511.36578226095844e-1550.8668106601845930.3760.086
TENM435.69952802176639e-1062.20715177236045e-1100.8607497201742720.8140.522
STON231.41840477111284e-1685.49279623247818e-1730.8521878674930870.5310.166
NECTIN335.40029790245025e-1692.09127440748567e-1730.8351843473905680.5320.171
ENSG0000025863131.28261582712522e-1294.96695127260668e-1340.8298880637874270.4960.176
FMN235.31276710248648e-742.05737795859756e-780.8290747930042590.7030.436
BAALC32.9643022956754e-1591.147931028802e-1630.8284383504496980.5090.154
GRIK431.49077834743126e-1155.77306411892987e-1200.8268192698221370.820.523
NTRK232.17878148209155e-1428.43736778101516e-1470.81817216635530.5430.178
PLXNA432.59430686490613e-1281.00464967854476e-1320.8030725571129790.4660.148
MT-ND447.53719786376828e-1492.91879249652181e-1531.32363767770190.9970.975
MT-CO141.04326348796457e-1544.04005533038212e-1591.275610308384260.9920.932
MT-CO245.20994855683515e-1322.01756130458705e-1361.217283643196410.9720.82
MT-CYB49.92671296844287e-1383.84413622291867e-1421.20416277896770.9840.895
MT-ATP645.83965319180255e-1442.26141547914748e-1481.193224740497040.9810.877
MT-CO348.5842798918296e-1173.32427676560802e-1211.109437452174190.9450.705
MT-ND542.35923626700371e-1199.13618195795884e-1241.027137419197340.9890.937
MT-ND249.60594440830278e-963.71991806076087e-1000.9595990259010710.9420.761
MT-ND144.93755558253472e-1061.91207666906817e-1100.908459917385680.9430.712
MT-ATP849.54116070503944e-593.69483046316828e-630.7752509055844740.5730.333
MT-ND4L41.68644948326896e-626.53080386968579e-670.7455015434901930.5650.303
MT-ND342.22759580117715e-678.62640204924738e-720.7268477324584890.5730.314
MT-TV42.45013301697525e-659.48818114462012e-700.6938899811659670.6380.365
ENSG0000028044149.11183187009782e-083.52857215276994e-120.6689625849079950.9870.981
FGF1247.64946172479013e-302.96226686472917e-340.4731224146650930.9370.817
PVT141.50545298269791e-255.82989188978008e-300.4213946309823160.9210.791
REV3L45.45822487250987e-332.11370672366103e-370.4052035660301760.9850.928
IGSF9B43.50694825880256e-141.35807158688091e-180.391947845444770.4860.337
MAP248.68506640550501e-283.36330651183248e-320.3858318477425020.9920.927
POLR2A40.003023737897245211.17094756505643e-070.3787928496230870.3810.302
DHFR41.48578133630424e-255.75371310964739e-300.3658295906419010.4560.275
MTSS145.48722411888309e-142.12493673038883e-180.3546029083776450.9680.868
SMOC147.99872920299233e-183.09752128063832e-220.3516690176401230.9230.817
RPL37A41.79320558980396e-076.94421868026161e-120.3466887433590370.3460.243
LINC0046145.75086657088643e-052.22703271149225e-090.3293348656294560.7970.687
FAM110B41.11563132115417e-154.32030097647126e-200.3126124303304220.9460.846
GRIK343.66781161651807e-141.4203661915804e-180.2913478555011850.9390.804
EPN243.94350528342608e-131.52712902583978e-170.2797984281780740.830.677
CCDC2641.58664351614403e-126.14430359038077e-170.2794104114579380.8520.693
ADAM2248.09258279425562e-113.13386624104698e-150.2728321864929040.8780.764
CADM241.66323495368923e-106.4409052150766e-150.2714698872534580.9930.974
MACROD141.03615110970865e-094.01251252646343e-140.2681558164689040.7160.56
DLGAP147.63717443531816e-132.95750859130162e-170.2680909358797070.9970.975
CMSS142.3303203702469e-109.02420466346631e-150.2658972434654190.810.68
CORO1C41.70683544676624e-146.60974885476605e-190.2645815307366770.6810.496
LHFPL342.03669486443793e-127.88713497439464e-170.2616748677661860.9970.954
NOVA141.4378102738865e-105.56794436698486e-150.2598257856416960.9810.934
KHDRBS341.61049955654879e-096.23668650640432e-140.2594846774886710.820.689
LDLRAD345.60682334865307e-112.17125173242965e-150.2565768868478040.9620.878
UST47.28677588016461e-082.82181616394865e-120.2517593478604530.8950.774
KCNIP45003.158468334498850.9650.29
MMP1651.48098644042583e-1555.73514479505027e-1601.459237236261360.9270.662
FGF1251.84303915967765e-1597.13720001424177e-1641.22690673266110.9850.813
DLGAP157.75202399932366e-1613.00198427732009e-1651.046059667608360.9970.975
LHFPL353.05924141963499e-1311.18469636356542e-1350.96789573905880410.954
KHDRBS358.52533791628532e-1183.30145138685874e-1220.9097016039350020.9210.681
KCNH157.99473550063959e-793.09597471271331e-830.8843872224859270.7820.493
EPHB157.25797827441747e-812.81066424289102e-850.8756950566936450.9310.812
CHST951.04196843358559e-804.03504021060909e-850.8682487942894830.7350.412
SOX654.88908226511389e-1181.89330529571076e-1220.8393386225131720.9850.853
CA1058.75801862446106e-783.39155738080822e-820.8277043366613050.5230.21
HIP159.93164154455131e-873.84604482227135e-910.7917092772454340.9060.669
MAP252.32087464810744e-1158.98762594627826e-1200.7820938616176540.9970.927
XKR452.74472188377227e-821.0628981465253e-860.7587378485819690.9370.714
PCDH1552.7622347196035e-831.06968002153255e-870.750541301827240.9590.814
LUZP251.98066691870053e-917.67016581613494e-960.7449412181661910.5420.202
CADM252.46965933155891e-929.56379712488443e-970.7011673080726530.9990.974
ADAMTS2051.98409059435099e-1047.68342405743326e-1090.6949103562218170.6370.258
SCFD258.11106293505757e-793.14102270652425e-830.6913624127995230.9390.749
CCDC17852.17750360994273e-488.43241919971624e-530.6847490696491060.370.151
COL20A151.27095784360389e-1064.92180553616501e-1110.6784418858425130.690.287
IGSF9B57.82490328198547e-823.03020690159372e-860.674175591885730.6870.321
EPN252.85133269903434e-761.10418336329409e-800.6695194746639950.9010.672
SLC44A151.74447855305717e-916.75552241434835e-960.6666849478872910.8760.562
SMARCC151.22706939927624e-954.75184680043466e-1000.6538500440407860.9840.847
CNTNAP251.52634424027241e-445.91079363463739e-490.64376838308040.880.753
REV3L58.01199072894966e-793.10265682877654e-830.6412788912443690.9970.928
KAT2B53.08491556469181e-781.19463871923937e-820.6315902400673740.7190.375
GRID254.21903886438294e-531.63382986654647e-570.6278604843120040.9040.742
UST55.06814340184416e-661.96264702081251e-700.6272392049577040.9430.771
PVT153.58078726834118e-641.386665866995e-680.6235459128901860.9470.79
TCF7L151.64426548018317e-686.36744561121158e-730.6199079191073960.8140.525
TPP257.38355848511885e-752.85929538981484e-790.6141350881346820.8630.591
FAM110B52.11196004733029e-758.17860065573437e-800.6111354257966310.9820.843
SEMA5A53.57871322137834e-461.38586268883489e-500.6098228507288270.9040.762
PID153.56277406219002e-611.3796902227433e-650.6007321595702390.8360.595
ETV158.85583872735622e-533.42943837948969e-570.5937734749720890.8320.626
MDGA259.52625291880231e-633.68905739797944e-670.5929425764071550.9420.807
SH3D1951.15460188669899e-694.47121514424732e-740.586339322208110.940.758
ATP13A454.64536791281683e-661.79892650459545e-700.5790549750508710.6650.322
MTSS151.49739640453527e-625.79869265590857e-670.572012613011180.9880.867
ELMO152.24542845391197e-598.69545929563554e-640.5701637172783380.8040.514
ST3GAL551.13600842448301e-364.39921165040083e-410.5673184745357640.6580.429
DSCAML155.0926130854318e-591.97212294676521e-630.5663683547733960.9910.921
DIP2B51.78526438273394e-666.91346622287861e-710.5603090707420340.9750.891
SERINC552.66673022562624e-561.03269574628286e-600.5561340039242880.8740.679
JMJD1C55.73253907162416e-742.21993535670688e-780.5506130382365850.9940.966
GALNT1355.00922927103592e-491.9398324249839e-530.537331410016760.9520.831
ADGRL354.90107241943964e-721.89794850305528e-760.5370686675104510.9970.934
LRRN159.54248977404537e-573.69534514736683e-610.5354761425299490.830.575
ATAD26001.919918026269160.9360.278
RRM26001.909292985476950.8750.094
DIAPH36001.905221295381930.8690.083
RFC36001.716249735911650.8720.237
MIR924HG6001.518837618227360.6670.088
BRIP16001.463678356280250.8040.139
APOLD16001.432119793974270.8190.17
CENPP62.93008685821876e-2171.13468104334073e-2211.319304959715310.9030.435
FANCI63.67680268498418e-2941.42384799790271e-2981.231679065902570.7790.199
RTKN26001.173444942073620.6820.061
SPC256001.14848799325790.7080.093
POLQ6001.13729154724640.6590.041
MMS22L66.60087982489905e-1982.5562017677648e-2021.131502629300320.810.321
TBC1D3163.15195222427272e-2891.22059877793932e-2931.119187031846760.7370.167
CENPK63.79396709172329e-2131.46922011064682e-2171.116428348285260.8190.306
MELK6001.059535790585570.6590.061
EZH269.6407741990287e-1743.73340595555462e-1781.045983022253790.8780.453
DLEU262.35259175467321e-1329.11045097267248e-1371.012988187561330.9360.731
KIF156001.012338537453630.6320.062
GRM863.024281296782e-1071.17115799743717e-1110.970993840469170.7180.337
DTL66.64474771347378e-2672.57318968108809e-2710.9606892712786540.6510.132
ENSG000002275986000.9511475256152080.6030.045
CIT6000.9499248943193550.6510.102
CDK5RAP266.34250180740075e-1422.45614444774068e-1460.9221934542717620.8550.472
CENPI6000.9191497035326510.6230.085
KNL16000.8941798132215970.5980.047
SNHG3162.51354844017988e-2269.73375843310181e-2310.8913130276623520.5130.089
RAD54L6000.8909404145510.5650.048
FANCD26000.8595396681585670.6070.087
KIF116000.8581395700152920.5770.059
BLM6000.8410344026819550.6150.097
DOT1L62.6286027420597e-851.01793081441339e-890.818129466301880.8160.53
LINC0157261.93006817881992e-1517.47422134848749e-1560.8162162393471920.5980.179
KIF4A6000.8157306849531530.5330.047
MKI676000.8154325275947220.5480.054
ENSG0000026738361.26570663187231e-2154.90147013078384e-2200.8076017729604770.6130.14
RAD1861.51447091853848e-1255.86481399736081e-1300.8034925649273460.680.274
PRKD161.10776715903933e-814.28984687696753e-860.8002227268161550.9090.681
BUB1B6000.7926604681121850.5280.042
ENSG0000022490561.39768378780017e-1075.41255387755167e-1120.7903838443858510.810.418
SMC461.53155408012438e-2895.93096882672185e-2940.7903337574809110.5680.084
SOX2-OT62.13044246089331e-898.25017411181237e-940.7822019882401250.9820.889
FANCC63.47292044940914e-1151.34489426070137e-1190.7791250681045520.7140.313
KIF246000.7747277363346770.5110.056
NCAPG6000.7695920063696420.530.049
ZGRF161.93849374746317e-2417.50684950417524e-2460.7664979681971250.5660.106
PRIM269.24400708670537e-1193.57975722677666e-1230.7534439632223830.6680.267
ROBO161.7425469400143e-326.74804221048795e-370.7532203192083530.7950.675
ATAD562.34588151657908e-1889.0844654632656e-1930.7275762637433940.5720.136
ENSG0000028367468.23659476118126e-953.18963511643932e-990.7187102422172420.8630.508
ASTN27002.700416886096860.9120.243
NRG37002.562353659834660.8630.164
KCNN37002.517326184472950.8620.126
CADPS7002.512669293084690.720.084
ADGRV17002.464834573563660.880.235
NTRK27002.066739620070550.7940.176
PTPRT7002.024579910376440.7370.093
SLC4A47001.91180646386890.7660.113
TNIK77.49722220124493e-2632.90331185425587e-2671.740382733323230.8230.246
ENSG0000025160078.8795925285421e-2303.43863707878329e-2341.727664437044820.4810.077
FMN271.00516575918181e-1633.89252123758592e-1681.702816884655920.8480.438
DCLK171.31824376976826e-1795.10492107721124e-1841.693032005518930.730.267
PARD3B78.97679499746001e-2783.47627889767262e-2821.678603077162170.7770.206
SORBS175.59568353802227e-2152.16693782210521e-2191.59765446862210.8280.33
SPARCL17001.5749448406280.7660.162
EEPD176.99182593982261e-2552.70759630555033e-2591.546356042290530.7770.195
POT1-AS173.29768699775847e-1931.27703481305754e-1971.529592365104950.6580.193
PPP1R9A-AS176.0920045668246e-2762.35913897177888e-2801.52027523430210.4860.06
ITPR271.78550162776953e-2916.91438495825245e-2961.518530342620340.6710.123
ERBB471.65216094111508e-2126.39802091590862e-2171.516837661520430.7390.233
NTM71.27367431348455e-1904.93232511127504e-1951.506457520784640.8570.351
KCND272.68266542720105e-2111.038866679782e-2151.481670182586590.7840.243
LIFR74.09464871924164e-2231.58565957450398e-2271.475545222235240.7150.201
GABRB175.47285293105457e-2502.11937146383246e-2541.461510487804830.5280.086
ARHGEF47001.418836931457880.6420.109
PDE8A72.23909951309278e-1318.67095036631214e-1361.412211405344850.8580.569
SGCD71.62695348241513e-2526.30040460990253e-2571.394478592039330.6290.13
FRMD576.84571638789856e-1602.65101513685418e-1641.388679415788370.9020.499
SLC1A376.24896689969811e-2202.41992289807463e-2241.388097265957670.8870.318
TENM476.2587192605611e-1242.42369951615269e-1281.37130645878230.8580.532
DCLK271.95831575615558e-1867.58361056482819e-1911.36841178988280.9730.649
CLU7001.348083527297450.5310.061
L3MBTL478.43775896419126e-1913.26753629097752e-1951.343464156699920.6070.153
ENSG000002877047001.319065808278690.5190.04
ATP1B27001.297764386183230.6290.079
TNC78.15443414164243e-2333.15781827891509e-2371.261679087506150.6040.116
TNRC6A72.30461564228758e-1468.92466267392471e-1511.251108446956430.8870.577
DTNA73.16366764161898e-1371.22513559292839e-1411.249413327657420.9290.643
DGKG79.50030177716978e-1713.67900777491762e-1751.244311787562470.4790.098
LRRC4C73.69649512826872e-1121.43147392954681e-1161.213915239509150.7930.459
GABBR272.98584288540262e-1101.15627265825141e-1141.213357907606510.4320.121
CACNB271.5430283398352e-1445.97540308963016e-1491.199393625858870.6710.234
ADCY273.33792332792979e-2281.29261639930674e-2321.193195510545940.6850.147
SPON172.23226535518753e-1128.64448497536122e-1171.191519086617110.730.365
LRIG172.93584445589386e-1621.1369106826836e-1661.19098621219740.9460.572
PRRX172.5028418018495e-2259.69229679684585e-2301.177509859713490.4770.076
ATP13A472.61424653865934e-1291.01237135060192e-1331.162252645840120.7280.321
PCDH779.33199226405688e-2673.6138296340692e-2711.140502421284920.5580.085
ENSG0000028367476.29858973710782e-1092.43913942497301e-1131.136923913551590.840.513
RFX471.50332180647925e-1255.82163887417901e-1301.135629599290240.6850.279
DSCAM87.96503641339578e-2623.08447369143623e-2663.336231740517140.9810.481
KCND28002.714885783534920.9620.245
TNR82.17865025696933e-1958.43685960953152e-2002.28634226600320.9450.501
DPP683.30750498803593e-2091.28083684623627e-2131.966619595792410.882
CSMD385.78359399225516e-1822.23970646023125e-1861.943901338503360.9780.659
EYS85.70023561951243e-2312.20742579077273e-2351.663815982873550.7750.184
PCDH1581.20413615250802e-1544.66303741822416e-1591.580352055668620.9980.817
CDH138001.535701276895610.7780.127
PTPRZ183.92238684008915e-1671.51895087328705e-1711.4915738418843810.947
DCC87.86646110338357e-1803.04630023753381e-1841.468123554903460.7940.223
TPTEP181.63715927925315e-1806.33992672909093e-1851.438297527903890.4740.07
CSMD183.15764177630037e-1191.22280206649126e-1231.396883203487770.9550.628
ENSG0000027825483.25367759222023e-3031.25999209705311e-3071.375539966884160.6290.077
NTRK384.72373545439831e-1771.82927446632781e-1811.374419485492520.8010.233
CDH1084.92821249710199e-2861.90845854358595e-2901.369655573068850.6890.102
LRRC4C82.57047736153587e-859.95421663453459e-901.333066294238890.8090.465
ANKS1B87.72886829302108e-1042.99301719127177e-1081.307364067379290.8230.395
MMP1681.03555629763043e-904.01020910672824e-951.302292384098580.9230.671
HIP181.12986653716366e-1074.37542708888842e-1121.291421248795860.9520.675
ENSG000002867788001.290136707295130.6580.022
ARPP2181.64815376835342e-1906.3825030722744e-1951.28882637905230.7560.192
GRID284.89335374627162e-1111.89495943394324e-1151.269137081563070.9810.743
DOCK1088.60305662274274e-1383.3315480861026e-1421.246711163299630.8880.389
DACH281.10849266978647e-2054.29265642948715e-2101.207375448861890.6750.132
RAPGEF489.10336445607581e-923.52529313250815e-961.190614848777110.8280.425
PTPRT83.6183972301396e-1831.40123038769299e-1871.183741150621050.6150.113
FERMT187.06559146676746e-1042.73616212940691e-1081.166246439745310.7890.358
GRIK485.1241949083793e-1051.98435306059687e-1091.148597045571870.8920.539
RAB27B81.69065623430249e-2396.54709458352046e-2441.134717908958090.5020.059
PCDH788.78610833880338e-2333.40243516973372e-2371.105740063622760.620.092
NCKAP585.2272427063681e-992.02425849295903e-1031.105505641062080.9640.628
ETV183.68354926393248e-821.42646062190004e-861.08954187622550.9090.628
GRIA486.14982719098518e-772.38153087983007e-811.087359939769240.9210.732
SHC381.51804647790207e-635.87866041088205e-681.075083218330910.4210.137
GALNT1381.67947965793532e-1016.50381310434618e-1061.072040851632410.9740.834
BRINP381.17348161663984e-2114.544327214653e-2161.039104237914780.5170.07
NTNG186.16925432906034e-2322.38905407158748e-2361.036666436656690.5570.075
IL1RAPL185.26903259105083e-972.04044169579477e-1011.018665824140610.8730.505
PTPRD86.53996476345998e-1112.53261230819811e-1151.013563139009530.7630.276
SPHKAP82.63415585626012e-1711.02008126718821e-1751.013215532206520.5070.085
CDH2081.69231022591007e-846.55349969372293e-891.010242581481860.9450.675
CHST1187.94316746352547e-693.07600490397145e-731.004613428148530.9350.706
LRRTM389.41844312439464e-1663.64730787452838e-1701.000100416211280.5790.113
OPCML82.8201036820499e-301.09208987416253e-340.9972847956306230.4780.243
MAPK1082.02519807508801e-837.84261346508154e-880.9731980846498890.8850.571
XYLT187.54710879647227e-492.92263052181089e-530.9709885236175260.5690.262
LSAMP87.09180074679164e-1012.74631171699324e-1050.96379990328483710.954
CNTN183.14571867227244e-891.21818482448687e-930.9476696245631830.6960.262
SLIT186.8660851087052e-572.65890295810138e-610.9406493331226540.7250.377
HDAC484.19466263069535e-771.62439012922408e-810.9376572512267480.730.331
ATP10A9004.230595172065040.9690.026
DOCK99003.656331192310920.9610.077
ABCB19003.591364767355520.940.042
ST6GALNAC39003.544481930889610.9530.197
MYRIP9003.219601801879980.9350.062
ANO29003.032303418466670.6920.024
SLC39A109002.974309645753190.8860.205
FLT19002.93936980479890.8450.021
ABCG29002.901398171905060.870.018
EPAS19002.897301907119190.8910.026
MECOM9002.813243184890570.8260.035
PTPRB9002.666135823646140.8550.017
MIR3667HG9002.665809896466450.8630.051
SAMD129002.6599506642730.8370.07
GALNT189002.533363674774530.8650.147
PLEKHG19002.498646875612080.8320.039
TTN9002.470729063193380.5830.03
TACC19002.450085888220010.870.143
MYO1093.04161019225668e-1601.17786864123327e-1642.368885688742770.8830.478
A2M9002.36025557100040.8240.059
NEAT193.47507330841486e-2161.34572795895708e-2202.318450549925540.9480.378
LEF19002.306402074924730.7620.046
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COL4A2147.4837124331108e-682.89808017391891e-721.867286198858630.6720.158
SLC19A1141.01818728460109e-193.94294731286486e-241.863772667739870.6260.356
FRMD3144.3295523260662e-1221.67662638967827e-1261.858342601890910.6560.084
COL4A1141.52261862075423e-1505.89636611065417e-1551.853795793418180.6410.064
RBPMS141.74380748671074e-1876.75292369868234e-1921.839585457129810.6720.055
IGFBP7142.68014264614517e-1821.03788972859279e-1861.827681083591430.6560.052
PTEN141.26065425198216e-654.88190470503875e-701.826279190892930.7250.208
CARMN14001.816129687081470.6490.003
RGS5143.0086546565155e-2511.16510655482148e-2551.806952923365090.5570.026
LHFPL6141.57318002024181e-556.09216597700426e-601.768904976696220.6870.185
ZFHX3141.20434180548276e-454.66383381281321e-501.759013746000430.7860.349
PTPRK148.06504857924363e-403.12320357016754e-441.741634297908270.6950.284
BGN141.39765703960197e-2185.41245029470614e-2231.659991246545750.5570.03
COLEC12147.72546167286185e-1992.99169797190948e-2031.654885431816750.5570.034
RNF152148.56221783121497e-913.31573319568407e-951.651694135580970.580.087
NID1141.55627853331323e-906.02671468579649e-951.638919071769350.6790.121
EPS8148.63580357699337e-513.34422939898284e-551.628685049448530.6560.196
GRK5141.39527984940658e-765.40324458585981e-811.617316955719830.6640.136
COL18A1141.43657157017147e-735.5631474661018e-781.603320392506050.580.101
LAMA4142.54798010385069e-1029.86709562735039e-1071.602123338708750.6260.086
FKBP5148.01935042200335e-873.10550688223806e-911.591283913863510.6260.097
CDH6141.7905008376482e-2976.93374448223754e-3021.587703567862530.5420.019
ENSG00000243620143.66051265343754e-1471.4175396559027e-1511.584455303201150.550.046
SLC1A3142.62520509122548e-471.01661506843724e-511.567269664658460.870.349
UTRN145.15291524900697e-571.99547506060759e-611.551662674854290.6560.167
RBFOX1152.52941002859372e-1419.79518269989434e-1463.720086376901060.9570.17
NRXN3151.83592294373818e-1257.10964234882926e-1303.420952059746560.8870.154
OPCML151.11558040391627e-874.32010379861467e-923.018935580714540.9040.246
RBFOX3152.10750208673383e-2908.16133712865985e-2952.687476277186220.7650.04
ASIC2154.74562863353188e-2711.83775263661538e-2752.647447102755010.6780.033
DLGAP2158.20465397105007e-2263.17726599196455e-2302.627687435049650.7570.053
SYT1159.98412558651513e-1603.86636935542544e-1642.535589868658140.8520.107
KCNIP4151.98216699433414e-347.67597488415035e-392.403150731960790.7740.338
MYT1L15002.345122375222840.7480.027
GRIP1151.27589629854199e-2074.9409297856252e-2122.263576783774340.6170.036
GRIN2A15002.263181727184310.6870.025
PTPRD151.17069923434308e-444.53355239260767e-492.206966453852220.7740.293
RYR2151.09348640473209e-1874.23454441672962e-1922.184897565417420.7130.057
NRG3151.64154575557861e-866.35691343212875e-912.18056689594260.8960.203
ROBO2155.28041214281176e-372.04484844627338e-412.154050353795980.8960.466
IQCJ-SCHIP1153.57750198125248e-551.38539363406749e-592.115633147457620.5130.094
KAZN154.21210633444825e-491.63114523271822e-532.106539539911320.9910.527
NKAIN2157.11835210318298e-932.75659377422568e-972.065618100136360.6780.099
TENM2151.93031588360024e-1017.47518058939797e-1062.064968731370210.7040.106
TMEM132D151.04946274451187e-2004.06406205519061e-2052.00692594764730.670.046
GRM5153.00091246927355e-2081.16210837984492e-2121.972717718379660.6780.045
RALYL152.4674004308926e-689.55504949422064e-731.91613532984310.6090.112
GALNT17151.26768471525699e-1474.90913029182121e-1521.909254052395140.6520.058
FRMD4A155.31312828660755e-572.05751782775338e-611.898944265852240.870.314
KIRREL3151.96384486796228e-517.60502214290471e-561.852610237251460.4960.086
SNHG14154.65577687648094e-461.80295739320797e-501.8296513680892110.734
ATP2B2157.58380244179721e-702.93684019741982e-741.783386550817420.7130.159
CACNA2D3151.12649651319232e-584.36237661461614e-631.760019137186520.6610.152
RGS6151.074001881918e-1304.15909027579288e-1351.758976628512210.5220.041
LRRC7151.32885566205091e-885.14601580781053e-931.748209203473040.6350.091
ANKS1B151.3601712039907e-435.26728576846492e-481.747358726120010.8960.41
CELF4154.56024605325965e-481.76596292191444e-521.744495992834540.7220.218
AGBL4153.23938867859809e-651.25445869132095e-691.730086558100270.7570.19
GABRG3153.09412003244807e-2881.1982031647942e-2921.722403655370350.60.023
GRM7151.1636544894652e-1374.50627150007824e-1421.711418439341070.5650.047
HS6ST3157.91147207582065e-873.06373081199731e-911.689593180948240.5220.063
CNTN5154.41046246150973e-451.70795897514221e-491.688835929345270.4430.082
SHISA9157.01873325627443e-1812.71801620891238e-1851.671317377590450.5740.036
FRMPD4155.39115446160778e-1512.08773359470541e-1551.662625950599170.5480.04
KIAA1217154.07120675512101e-141.57658163463618e-181.657200475540960.3830.134
CCSER1158.72190735001001e-923.37757322929559e-961.656680371281460.6780.107
CHRM3151.00787562396743e-1793.90301523435478e-1841.643799282707250.5650.035
KALRN156.05407649318753e-402.34445126173858e-441.626178900935480.9570.538
GRIN2B152.73362776009677e-2031.05860192855082e-2071.619936385648470.6350.039
DPP10152.30509399723299e-208.92651511146262e-251.600226220883140.7480.395
DLG2157.56907694237544e-452.93113772310554e-491.5985447076252310.767
RGS7152.02823443643365e-307.85437182524744e-351.591700547179410.670.276
KHDRBS2155.67174969034088e-1992.19639456699101e-2031.58750852125950.5740.032
CNTNAP5157.01001898901961e-702.71464159432274e-741.581208549717420.5570.089
NRG1153.16146592785085e-641.22428297558411e-681.576719524226610.4520.061
SLC1A2161.27912012865204e-924.95341412172112e-973.987595609356830.8970.2
HPSE2161.35496363398755e-2655.24711936640803e-2703.294629299574880.8450.049
ADGRV1168.04886206974102e-653.11693531725246e-693.287903217521990.9070.273
GPC5162.58304463296474e-731.00028836036276e-773.073895495615690.7840.169
OBI1-AS1167.11843118945906e-1012.75662440051855e-1052.664085377666550.8450.14
NTM167.33880944309283e-572.84196624834172e-612.637432910038810.9690.379
CLU161.10859543907327e-1224.29305440527155e-1272.555787251095210.7530.086
SLC1A3163.33364957259921e-571.29096138039702e-612.504010510664250.9590.35
SLC4A4168.16990424126372e-903.16380910090374e-942.437648388005240.8450.15
SPARCL1164.98501322700025e-781.93045472137252e-822.409719990145960.8760.196
KCNN3161.46635979922662e-845.67850288203006e-892.356700671653150.8870.169
ATP1B2161.02608292385285e-893.97352330810846e-942.28600261122030.7320.11
DPP10164.80100773159058e-181.85919828509104e-222.11634950111240.7530.396
NTRK2162.80620542829973e-601.0867077521201e-642.095746856216040.8250.212
ATP1A2162.15148092983391e-3018.3316459351505e-3062.050470167871990.7420.028
TNIK168.00190731124723e-403.09875200838293e-441.97433131058680.7940.281
DGKG167.42469816686526e-902.87522680047449e-941.960318192539510.7630.117
GABRB1161.09668387619811e-874.24692667853506e-921.951510584411880.7320.11
LINC0029916001.947646607360080.660.02
CADM1162.74766279349751e-221.06403701874202e-261.814672668902360.6490.295
TNC161.97350015929774e-627.64241242031422e-671.810485063820260.7320.143
MGAT4C161.21420948428331e-1204.70204656423852e-1251.781603786528480.680.068
NRG3165.35729784878301e-412.07462256468381e-451.764509538971990.7420.207
AQP4164.39019473108683e-2601.70011026259026e-2641.744447738000740.5980.021
ENSG00000280441164.51737991664568e-221.74936293871575e-261.728355855546870.990.982
PCDH9162.35979832532096e-389.13835853820611e-431.72471994867630.9590.495
DTNA162.58752931408194e-351.00202506063662e-391.704699848994190.9690.659
PRKG1163.11350871406679e-821.20571146422445e-861.664967439463090.6190.081
SLC39A11167.51056391844318e-262.90847845658645e-301.662790009761410.7530.364
IRAG1166.25193740240305e-1952.4210732302223e-1991.616413646463430.6910.042
SHROOM3161.32779789376505e-2305.14191958240736e-2351.596256258505710.5980.025
ITPR2169.54131886985975e-423.69489171275985e-461.584678412480140.6490.156
MAPK4161.27523406700703e-624.93836528291456e-671.573908490405340.6390.117
CABLES1162.49580056700953e-1089.66502949699698e-1131.546648492750820.5360.046
RYR3166.89869392573171e-1052.67153077711022e-1091.521947432673920.4640.034
CARMIL1163.63766900369937e-1141.4086934142816e-1181.521827970591190.5880.052
PREX2162.11835196565824e-568.20335346651529e-611.483449489317740.5670.092
ARHGAP26163.57612568045228e-511.3848606592775e-551.481632959453940.7010.149
ENSG00000286288168.12114884860364e-1373.14492849343749e-1411.479040393715340.6190.049
TAFA1161.84953707021396e-807.16236328162476e-851.475658331482830.5570.065
ACSBG1162.12907955272469e-1228.24489622710253e-1271.471114761959540.5880.049
ABLIM1161.47217459862727e-305.70102079009904e-351.465271057196920.7320.275
FMN2164.28688233169299e-251.66010236289083e-291.439938507822480.8870.461
NAV3161.61319183392567e-426.24711239563827e-471.434024017634570.6490.157
CACNB2166.72018945305155e-322.6024046210942e-361.418157253368270.7420.259
SHANK2161.03958762306052e-224.02582048197543e-271.390712522645350.680.287
NPAS3161.21980082361138e-244.72369911943375e-291.380543516145430.990.733
PITPNC1166.74387933051293e-232.61157856581843e-271.374204719157960.7840.413
CDH20168.2072804986814e-223.17828311918886e-261.370277584892560.9380.686
MAP3K5168.18632270859822e-713.17016717987771e-751.369304296781450.6290.091
Gene Body Coverage


geneBody
This plot illustrating the coverage profile along the gene body. Scaleing all transcripts to 100 nt and calculates the number of reads covering each nucleotide position. The coverage was calculaterd for each position as NUMi/(total NUMs)). Here, NUMi denotes the number of covered reads of position i.
Saturation


Left
The plot shows the Sequencing Saturation metric as a function of downsampled sequencing depth in mean reads per cell. Sequencing Saturation is a measure of the observed library complexity, and approaches 1.0 (100%) when all converted mRNA transcripts have been sequenced. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.
Right
The plot shows the Median Genes per Cell as a function of downsampled sequencing depth in mean reads per cell. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.